منابع مشابه
Human recombinant laminin-binding protein: isolation, purification, and crystallization.
The mRNA of the precursor of laminin-binding protein (LBP) was isolated from a human embryo kidney cell line and cloned. The determined sequence of the LBP gene showed complete identity with the LBP genes isolated from human lung and large intestine cells. The human LBP was expressed by E. coli cells, and it was purified using Ni-NTA-Sepharose chromatography. The mobility of the homogeneous rec...
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At the summer workshop of the Korean Endocrine Society held in 2016, some examples of protein experiments were discussed in the session entitled "All about working with proteins." In contrast to what the title suggested, it was unrealistic to comprehensively discuss all protein analytical methods. Therefore, the goal was to outline protein experimental techniques that are useful in research or ...
متن کاملAntigen Isolation and Purification
An antibody was raised against a protein isolated from the cytoplasm of mesothelioma cells. It was subsequently used in an immunoperoxidase procedure on formalin fixed, paraffin embedded tissue sections. A representative sample of benign and malignant tumours from all the systems of the human body was examined. All the tumours derived from coelomic surfaces (mesotheliomas of pleura, peritoneum,...
متن کاملisolation and purification of an antibacterial protein from immune induced haemolymph of american cockroach, periplaneta americana
background: antimicrobial peptides play a role as effectors substances in the immunity of vertebrate and invertebrate hosts. in the current study, antimicrobial peptide was isolated from the haemolymph of the american cockroach, periplaneta americana . methods: micrococcus luteus as gram-positive bacteria and escherichia coli as gram-negative bacteria were candidate for injection. induction ...
متن کاملIsolation and Purification of Dna
IMPORTANT NOTE: The smallest amount of contamination of DNA preparations by recombinant phages or plasmids can be disastrous. Many person-years have been wasted reisolating previously cloned sequences that contaminated preparations of DNA used to create recombinant DNA libraries and many researchers have been embarrassed to find that the “extra” genes they found on their Southern blots were act...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2009
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113061